Category: Enzyme
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Proteolytic Characteristics of Cathepsin D Related to the Recognition and Cleavage of Its Target Proteins
Cathepsin D (CD) plays an important role in both biological and pathological processes, although the cleavage characteristics and substrate selection of CD have yet to be fully explored. We employed liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify the CD cleavage sites in bovine serum albumin (BSA). We found that the hydrophobic residues at P1 were […]
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Preferential production of RNA rings by T4 RNA ligase 2 without any splint through rational design of precursor strand
Circular single-stranded RNAs (C-RNAs) are RNA rings without terminals, which are promising for many practical applications both in vivo and in vitro. Recently, for example, thousands of circular RNAs (circRNAs) have been found almost in all kingdom of organisms, and their biological roles as miRNA or RNA inhibitors have been reported. Many circRNAs have also […]
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Design of a substrate-tailored peptiligase variant for the efficient synthesis of thymosin-α1
The manufacture of long peptides using conventional chemical strategies is still very challenging and the technology used has remained almost unchanged since Merrifield developed the solid phase approach in the 1960s. Solid-phase peptide synthesis (SPPS) is characterized by an exponential decrease in the crude yield and purity as the peptide length increases: coupling and deprotection […]
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Peptiligase, an Enzyme for Efficient Chemoenzymatic Peptide Synthesis and Cyclization in Water
There is a large and growing interest in pharmaceutical peptides over the past decades, with about 60 approved peptide drugs currently on the market and some 140 candidates in different phases of clinical development. By 2018, the worldwide peptide drugs market is expected to reach $18 billion. The importance of peptides is also recognized in […]