Product Name: PepFect 6
Synonyms: PF6, PF6, Stearyl-Ala-Gly-Tyr-Leu-Leu-Gly-Lys(QN4-MAP4)-Ile-Asn-Leu-Lys-Ala-Leu-Ala-Ala-Leu-Ala-Lys-Lys-Ile-Leu-NH2,
PF6 is modified by covalent attachment of trifluoromethylquinoline-based moieties via a lysine tree in the Lys7 position.Stable PF6/siRNA nanoparticles enter entire cell populations and rapidly promote endosomal escape, resulting in robust RNAi responses in various cell types (including primary cells), with minimal associated transcriptomic or proteomic changes. Furthermore, PF6-mediated delivery is independent of cell confluence and, in most cases, not significantly hampered by serum proteins. Finally, these nanoparticles promote strong RNAi responses in different organs following systemic delivery in mice without any associated toxicity. In comparison to TP10 and PF3, PF6 is much more potent delivery vector for siRNA in vitro and in vivo, presumably by trifluoromethylquinoline promoted escape from endosomes by osmotic swelling and thus decreasing endosomal breakdown of CPPs and cargo by delaying endosome acidification.