New phosphorylating reagents for deoxyribonucleosides and oligonucleotides

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New phosphorylating reagents  buy Lyrica canada pharmacy 1 and  buy Pregabalin online usa 2 were prepared in three steps from 4-hydroxybenzaldehyde. They showed good efficiency in the solid phase synthesis of 5′-phosphate monoester nucleosides. End-phosphate DNA sequence synthesis demonstrated the efficiency of the new reagents ( http://pacificindustrialmarine.ca/wp-json/wp/v2/pages/26 1 and  2) according to the general procedure of automated DNA synthesis. The oxidation of P(III) to P(V) and the removal of benzyl protecting groups were achieved in a single step by treatment with a 0.02 M I2/pyridine/H2O solution. Due to this one-pot treatment, it is possible to use the phosphorylating reagents (1 and 2) for the synthesis of base-sensitive ODNs. The reagents 1 and 2 are unique among phosphorylating reagents.

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